电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进骨髓的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：深入探讨紫外光是否可通过转化介素-β（TGF-β）-内源性的上皮-间质转换 (EMT)来促进抗体的摧残迁往。使用总额2Gy(60)Coγ两条路线照射源自人类器官的6种抗体，记录与EMT相关的变化，这仅限于分别利用显微镜技术开发，受体质印迹方法，免疫荧光技术开发，样试验和Transwell鲍洛通试验来观察并监测受体分组织形态，EMT标明，摧残迁往灵活性等。改用酶联免疫吸附法监测这些抗体当中TGF-β受体总体，利用相当多类固醇SB431542来评估TGF-β信号通路在紫外光EMT当中的作用。经过总额为2Gy照射的抗体当中共存间叶受体的表达，与真照射分组相较其上皮标明减少，间叶受体标明增加，同时其摧残转移灵活性增强，TGF-β受体总体也大大提高。有利于发现由A549紫外光作用于的EMT可通过对TGF-β信号可抑制发生逆转。这些结果表明TGF-β内源性的EMT在紫外光作用于增强抗体摧残转移灵活性当中起着关键。

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